Download Advances in Biochemical Engineering, Volume 003 by T.K. Ghose ,A. Fiechter, N.Blakebrough PDF

By T.K. Ghose ,A. Fiechter, N.Blakebrough

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LB__3_ . . . . . . N J - ! 2_ . . . . . I i I I I ! ~'_~ '" . . . . . . . . . . . . . . • Fig. 20. , 1971) a genetic analysis as loci responsible for the biosynthesis of the antibiotic. The important genes responsible for the formation of tetracyclines may thus belong to different, frequently independent, metabolic pathways. A common feature of these loci is the final effect of their action-provision of intermediates for the biosynthetic process. Van~k et al. e.

I - B2 I i ! I ,o I \, A . . . . . . . LB__3_ . . . . . . N J - ! 2_ . . . . . I i I I I ! ~'_~ '" . . . . . . . . . . . . . . • Fig. 20. , 1971) a genetic analysis as loci responsible for the biosynthesis of the antibiotic. The important genes responsible for the formation of tetracyclines may thus belong to different, frequently independent, metabolic pathways. A common feature of these loci is the final effect of their action-provision of intermediates for the biosynthetic process.

This second class contained also up to 90% of the isolated mutants; it thus appears that only a small number of mutants are really blocked in the proper biosynthetic pathway. Genetic Problems of the Biosynthesis of TetracyclineAntibiotics 35 c) Interspecific Cosynthesis In cosynthetic experiments designed by McCormick et al. ( 1961) a certain possibility ofinterspecific complementation was found; thus, for example, formation of OTC was observed in mixed cultures of S. aureojaciens and S. rimosus, and formation of CTC during combined cultivation of S.

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