By Cole, James L
Analytical Ultracentrifugation, the most recent quantity in Methods in Enzymology, makes a speciality of analytical ultracentrifugation. The scope of this method has significantly extended lately as a result of advances in instrumentation, algorithms and software program.
This quantity describes the newest concepts within the box and within the functions of analytical ultracentrifugation within the research of macromolecules, macromolecular assemblies, and biopharmaceuticals.
- Timely contribution that describes a speedily altering field
- Leading researchers within the field
- Broad assurance: instrumentation, uncomplicated concept, information research, and applications
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Extra info for Analytical ultracentrifugation
It is reasonable to expect different noise levels at different concentrations on different instruments; hence, we compared the root mean square deviations (RMSDs) obtained from the degenerate 2DSA analysis of the iterative refinement step, which produced random residuals in all cases. We plotted the RMSDs obtained for different concentrations from each of the six conditions: (a) BeckmanCoulter XL-A measurements at 258 and 278 nm, (b) open AUC MWL measurements at 258 and 278 nm, and (c) fits from the decomposition results for DNA and BSA as obtained from the open AUC MWL instrument.
2 Analysis Results The results from a 2DSA generate a surface of amplitudes for the two fitted dimensions for each wavelength. These data can be shown either as a 3D plot (Fig. 2) or as a pseudo-3D plot where a color gradient is used to project the amplitudes into a 2D plane (Fig. 3). In UltraScan, model files for individual fits can be combined to generate a global view of each analyzed wavelength. Solutes appearing in the global model can be integrated visually in UltraScan, and the global model can be displayed either in a 3D viewer (see Fig.
Modeling analytical ultracentrifugation experiments with an adaptive space-time finite element solution of the Lamm equation. Biophysical Journal, 89(3), 1589–1602. , & Demeler, B. (2008). Modeling analytical ultracentrifugation experiments with an adaptive space-time finite element solution for multi-component reacting systems. Biophysical Journal, 95(1), 54–65. , Laue, T. , Langhorst, B. , et al. (2010). The open AUC project. European Biophysics Journal, 39(3), 347–359. Correia, J. , & Stafford, W.